Novel insights in membrane biology utilizing fluorescence recovery after photobleaching.

نویسندگان

  • Amitabha Chattopadhyay
  • Md Jafurulla
چکیده

25-NBD-cholesterol 25-[ N -[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)methyl]amino]27-norcholesterol 5-HT 1A receptor 5-Hydroxytryptamine-1A receptor 5-HT 1A R-EYFP 5-Hydroxytryptamine-1A receptor tagged to enhanced yellow fl uorescent protein DiIC 18 (3) 1,1′-Dioctadecyl-3,3,3′,3′,-tetramethylindocarbocyanine perchlorate EYFP Enhanced yellow fl uorescent protein FAST DiI 1,1′-Dilinoleyl-3,3,3′,3′,-tetramethylindocarbocyanine 4-chlorobenzenesulfonate FRAP Fluorescence recovery after photobleaching GFP Green fl uorescent protein GPCR G protein-coupled receptor NBD-PE 1,2-Dipalmitoylsn -glycero-3-phosphoethanolamineN (7-nitrobenz2-oxa-1,3-diazol-4-yl) p -MPPI 4-(2 ′ -Methoxy)phenyl-1-[2 ′ ( N -2′′ -pyridinyl)p iodobenzamido ]ethylpiperazine

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genetic tags for labelling live cells: gap junctions and beyond.

The availability of green fluorescent protein (GFP) as a tracer for observing proteins in living cells has revolutionized cell biology and spurred an intensive search for GFP variants with novel characteristics, additional autofluorescent proteins and alternative techniques of protein labelling. Two recent studies - one on tagging with tetracysteine motifs and labelling with biarsenic fluoropho...

متن کامل

Restricted mobility of membrane constituents in cell-substrate focal contacts of chicken fibroblasts

We studied the lateral mobility of membrane components in cell-substrate focal contacts using the fluorescence photobleaching recovery method. The measurements were performed on isolated substrate-attached membranes of chicken gizzard fibroblasts. The diffusion coefficients of a fluorescent lipid probe and rhodamine-conjugated surface proteins within contact regions (identified by interference-...

متن کامل

The biochemistry of RNA metabolism studied in situ.

In vitro assays have contributed important insights into the mechanisms of RNA metabolism in cells. A growing collection of microscopy techniques is allowing the measurement of macromolecular binding and complex formation in the context of a real cell. We will first discuss two of the more established techniques. Fluorescence resonance energy transfer (FRET) identifies binding partners, pairs o...

متن کامل

Measuring protein mobility by photobleaching GFP chimeras in living cells.

This unit describes fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) using commercially available confocal scanning laser microscopy (CSLM). Photobleaching is the photo-induced change in a fluorphore that abolishes that molecule's fluorescence. The different characteristics of green fluorescent protein (GFP) chimeras in a cell can be studied by FR...

متن کامل

Chromatin Dynamics in Interphase Nuclei and Its Implications for Nuclear Structure

Translational dynamics of chromatin in interphase nuclei of living Swiss 3T3 and HeLa cells was studied using fluorescence microscopy and fluorescence recovery after photobleaching. Chromatin was fluorescently labeled using dihydroethidium, a membrane-permeant derivative of ethidium bromide. After labeling, a laser was used to bleach small (approximately 0.4 microm radius) spots in the heteroch...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Advances in experimental medicine and biology

دوره 842  شماره 

صفحات  -

تاریخ انتشار 2015